KAHA Ligation - definitie. Wat is KAHA Ligation
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Wat (wie) is KAHA Ligation - definitie


KAHA Ligation         
  • Type I KAHA Ligation (unmodified hydroxy group)
  • Type II KAHA Ligation (O-substituted hydroxylamine)
The α-Ketoacid-Hydroxylamine (KAHA) Amide-Forming Ligation is a chemical reaction that is used to join two unprotected fragments in peptide synthesis. It is an alternative to the Native Chemical Ligation (NCL).
Ligation (molecular biology)         
  • sticky end]] ligation
TECHNIQUE FOR JOINING DNA MOLECULES IN MOLECULAR BIOLOGY
Blunt-end ligation; DNA ligation
In molecular biology, ligation is the joining of two nucleic acid fragments through the action of an enzyme. It is an essential laboratory procedure in the molecular cloning of DNA, whereby DNA fragments are joined together to create recombinant DNA molecules (such as when a foreign DNA fragment is inserted into a plasmid).
Proximity ligation assay         
  • '''Figure 1''': PLA starts with the binding of antibodies ''from different species'' to 2 proteins of interest, in this case protein '''*''' (star) and protein#
  • '''Figure 2''': Binding of PLA probes.
  • '''Figure 3''': Rolling circle DNA synthesis starts.
  • '''Figure 4''': Fluorescent probes bind to the amplified DNA.
  • '''Figure 5''': Fluorescence microscopy image showing interaction of the proteins in the cytoplasm. Nucleus in blue, PLA product in red.
BIOCHEMICAL METHOD TO DETECT PROTEIN INTERACTIONS
Proximity ligation assay (PLA); Proximity Ligation; RISH-PLA
Proximity ligation assay (in situ PLA) is a technology that extends the capabilities of traditional immunoassays to include direct detection of proteins, protein interactions, Extracellular vesicles and post translational modifications with high specificity and sensitivity. Protein targets can be readily detected and localized with single molecule resolution and objectively quantified in unmodified cells and tissues.